Composite

Part:BBa_K3803016:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-10-01)


This is a efficient xylose utilization composite part.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 805
    Illegal XbaI site found at 6464
    Illegal PstI site found at 2114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 805
    Illegal PstI site found at 2114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 805
    Illegal BglII site found at 1265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 805
    Illegal XbaI site found at 6464
    Illegal PstI site found at 2114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 805
    Illegal XbaI site found at 6464
    Illegal PstI site found at 2114
    Illegal AgeI site found at 1936
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization


Source

Scheffersomyces stipitis

References